The product is made up of a cellulosic matrix on which amino benzoic acid provides the ionic charge and hydrophobicity attached to it. The resin works to separate proteins with similar isoelectric points and hydrophobicity in broad conditions while using a pH from five to eight and conductivity up to 50 ms/cm. Originally introduced in 2016, the next generation of Pall Biotech’s CMM HyperCel mixed-mode resin is designed for large-scale drug manufacturing. The columns will be ≤45 cm in diameter with a range of configurable bed heights (5–30cm) and standard OPUS pre-packed column lead times will apply, according to the company. Similarly, Repligen will introduce a new proprietary method of packing ceramic hydroxyapatite resin in its OPUS pre-packed columns in September 2019. The company plans to introduce the system to the market in October 2019. With a flow rate range of 0.01 to 25 mL/min, an operating pressure of 0 to 5 MPa (50 bar, 725 psi), pressure, ultraviolet, conductivity, and pH monitors, and dimensions of 335 × 482 × 464 mm without accessories, the ÄKTA go is designed to simplify method creation, achieve desired purity with ease, and make the most of bench space in crowded laboratories. Developed for automated chromatography descended from fast protein liquid chromatography (FPLC) technology, the system supports routine lab-scale protein purification for affinity, size exclusion, and ion exchange chromatography. ÄKTA go is GE Healthcare’s most recent addition to the ÄKTA chromatography systems portfolio. Soon-to-be-released chromatography products The kits come with three lots of a given resin with different ligand densities (high, center, and low) and are available for Capto S ImpAct, Capto SP ImpRes, Capto adhere, Capto adhere Impres, Capto MMC, Capto MMC ImpRes, Capto Phenyl, Capto Phenyl ImpRes, and Capto Butyl ImpRes resins. According to the company, this aids in the understanding of process parameters and raw material attributes, which are key to developing successful control strategies and productive processes. The kits provide access to knowledge from resin development projects, enable risk assessments and process characterization of resin variability, and interplay with process parameter impact on process performance and critical quality attributes. GE Healthcare also introduced Process Characterization Kits to its catalog in July 2019. Chromatography process characterization kit MabSelect PrismA, MabSelect SuRe, MabSelect SuRe LX, Capto S ImpAct, Capto Q, Capto SP ImpRes, and Capto Adhere prepacked resins are available for use with these columns. The columns come equipped with a bed height of 20 cm and an inner diameter of 10 mm. The columns can be run on chromatographic systems, including the company’s own ÄKTA chromatography systems, and are prepacked to save time, improve reproducibility, and eliminate the need for packing expertise, the company says. Validation columns from GE Healthcare involve pre-packed scale down Tricorn 10/200 columns for process development and process validation.
The column is compatible with a variety of chromatography resins including those used for monoclonal antibody (mAb) purification, viral vectors, plasmids, recombinant proteins, and more. Introduced to the market in July 2019, the ReadyToProcess 600 mm inner diameter chromatography column comes equipped with bed heights of 100–250 mm. The pre-packed, ready-to-use column offers enhanced flexibility and productivity in clinical and commercial manufacturing by eliminating the need for column preparation, packing, and validation procedures, according to the company.
The ReadyToProcess chromatography column with 600 mm inner diameter is the latest addition to the ReadyToProcess chromatography column line from GE Healthcare.
The following products promise to improve and enhance the reliability of process chromatography.
Advances in process chromatography are necessary for accuracy and productivity in biologics manufacturing.
0 kommentar(er)
